How to calculate vitamin C concentration?

How to calculate vitamin C concentration?

Concentration is measured by calculating the volume of solution* used to reduce the blue color of DCPIP to colorless. Using a burette, the mixture of lemon juice, distilled water and glacial acetic acid is titrated in the DCPIP solution. The volume is noted when the blue color changes to colorless.

How do you test vitamin C with the DCPIP?

Suck up some of the clear liquid into a pipette, then add it, one drop at a time, to a test tube containing a light blue solution of DCPIP. If the extract is acidic, the color will change from blue to red. Keep adding more and see if the color disappears completely.

Can vitamin C be detected using DCPIP?

DCPIP can also be used as an indicator for vitamin C. If vitamin C, which is a good reducing agent, is present, the blue dye, which turns pink under acidic conditions, is reduced to a colorless compound by the ascorbic acid.

How to measure the vitamin C concentration of fruits?

Measure the vitamin C content of a fruit juice sample by measuring the volume of sample needed to decolorize a DCPIP solution. Calibrate the results by comparison with a known concentration of vitamin C.

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What is the concentration of vitamin C?

Total body vitamin C content ranges from 300mg (near scurvy) to about 2g [4]. High levels of vitamin C (millimolar concentrations) are maintained in cells and tissues, and are highest in leukocytes (white blood cells), eyes, adrenal glands, pituitary and brain.

What is the concentration of vitamin C in an orange?

Oranges contain 53 mg of vitamin C per 100 grams. A medium orange provides 70 mg of vitamin C. Other citrus fruits, such as grapefruit, tangerines and limes, are also good sources of this vitamin.

What happens when DCPIP is added to vitamin C?

Ascorbic acid reacts with DCPIP, changing color from blue to colorless. They react in a 1:1 fashion, so if a known amount of DCPIP solution reacts with the plant tissue extract, the amount of DCPIP used gives a direct measure of the amount of ascorbic acid present.

How does vitamin C reduce DCPIP?

DCPIP is commonly used as an indicator of vitamin C. If vitamin C, which is a good reducing agent, is present, the blue dye, which turns pink under acidic conditions and is reduced to a colorless compound by acid ascorbic.

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What is the concentration of vitamin C in orange juice?

Other results indicate a vitamin C content of 33 to 50 mg/100 ml for orange juice (Valencia) obtained by pressing the fruits [46].

Which orange juice has the most vitamin C project?

Minute Maid Original contained the most vitamin C. Bolthouse Farms came in second, followed closely by Springfield High Pulp, Minute Maid Country Style, Fresh Navel Del Monte, Tropicana Concentrate, Fresh Navel Victor, Springfield No Pulp, then came Tropicana Pure No Pulp.

How to calculate vitamin C concentration? Concentration is measured by calculating the volume of solution* used to reduce the blue color of DCPIP to colorless. Using a burette, the mixture of lemon juice, distilled water and glacial acetic acid is titrated in the DCPIP solution.

How to test vitamin C with the DCPIP?

Also, how do you test vitamin C with Dcpip? Fill the biurette with the standardized vitamin C solution (100 ppm). Use the graduated cylinder to transfer 20 ml of DCPIP solution into the 100 ml beaker. Release the vitamin C solution into the DCPIP solution, 1ml each time.

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How to calculate vitamin C in fruit juices?

Average values ​​should be calculated from replicates to produce a more accurate result. We can also determine the mass of vitamin C required to decolorize 1 cm³ of DCPIP as well as the mass of vitamin C present in fruit juice samples. We know that 1 cm³ of 1% vitamin C solution must contain 10 mg of vitamin C:

How to calculate the DCPIP of a fruit juice?

Close the valve as soon as the DCPIP loses its blue color and note the final value. Determine the volume of fruit juice needed to decolorize the DCPIP and record it in an appropriate table.